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. 2016 Apr 25;6:25051. doi: 10.1038/srep25051

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Copyright © 2016, Macmillan Publishers Limited

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Intracellular cytokine staining of young and aged NP Tet⁺ CD4⁺ T cells harvested 7 dpi and stimulated with PMA/Ionomycin. The graphs show the mean ± SEM of the frequency of NP-specific CD4⁺ T cells producing: (A) IL-2, (B) IL-10, and (C) IFNγ. The data are from 5 mice/group of one representative experiment of two performed independently. Statistical significance was determined by Student’s t test. ***p < 0.001; ****p < 0.0001.