Table 1. The kinetic parameters of the purified his-tagged LDH and LDH*.
| Enzymes | Specific activity | Kinetic parameters | |||||||
|---|---|---|---|---|---|---|---|---|---|
| NADH (μmol/min/mg) | NADPH (μmol/min/mg) | NADH | NADPH | ||||||
| Kcat (s−1) | Km (mM) | Kcat/Km (M−1s−1) | Kcat (s−1) | Km (mM) | Kcat/Km (M−1s−1) | ||||
| d-LDH | 1240 ± 38 | 49 ± 3 | 7801 | 1.40 | 5.57 × 106 | 821 | 4.55 | 1.82 × 105 | |
| d-LDH* | 1587 ± 52 | 3101 ± 47 | 8540 | 1.01 | 8.46 × 106 | 8643 | 0.257 | 3.36 × 107 | |
d-LDH, wild-type d-lactate dehydrogenase; d-LDH*, engineered d-lactate dehydrogenase. Data were relative to three independent measurements (±SD).