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. 2016 Apr 25;6:24868. doi: 10.1038/srep24868

Figure 6. Whole body analyses of PreI Tg #2 offspring.

Figure 6

(A) Expression of GFP protein revealed by immunohistochemistry with anti-GFP antibodies of PreI Tg #2 offspring by confocal microscopy. Upper and lower panel show WT and PreI Tg #2 tissues, respectively. Images were taken under the same instrumental settings (same laser intensity, etc). (Scale bar: 100 μm) (B). Immunohistochemical analysis of PreI Tg #2 brain with anti-GFP antibodies. Inset a: white arrows show astrocyte and GFP colocalisation. Inset b: white arrows show neuron nuclei and GFP colocalisation. (Scale bar: upper panel 100 μm, lower panel 25 μm) (C). Immunohistochemical staining of PreI Tg #2 testis with anti-VASA antibodies. Colocalisation of GFP and VASA is shown by white arrows. (Scale bar: upper panel 50 μm, lower panel 25 μm) (D). Immunohistochemistry of PreI Tg #2 spleen with anti-CD45 and GFP antibodies. Homogenous GFP protein expression in CD45-positive white blood cells was observed. Colocalisation of GFP and CD45 are shown by white arrows. (Scale bar: 25 μm) (E). Merged (Epifluorescence and Bright) images of fibroblasts from WT tail and PreI Tg #2 tail and ear. FACS analysis using fibroblasts from WT tail and PreI Tg #2 tail and ear. (Scale bar: 100 μm).