Figure 1. Effect of NH₄Cl treatment and low vitamin D diet on body weight of wild-type mice and of kl/kl mice.

(A) Photograph of male wild-type mice (WT) as well as male klotho-hypomorphic mice (kl/kl) without (left) or with NH₄Cl treatment (15 g/l in drinking water) without (NH₄Cl, middle) and with (LVD, right) additional low vitamin D diet. (B) Arithmetic means ± SEM of body weight (n = 12–30) of wild-type (WT, white bars) and kl/kl mice (kl/kl, black bars) either untreated (left bars, Control), treated with NH₄Cl solution (280 mM in drinking water) (NH₄Cl, middle bars) or treated with NH₄Cl and a vitamin D deficient diet (LVD, right bars). ***(p < 0.001) indicates statistically significant differences from respective wild-type mice; ^###(p < 0.001) indicates statistically significant differences from untreated kl/kl mice. (ANOVA).