Figure 2. Effect of NH₄Cl treatment and low vitamin D diet on plasma 1,25(OH)₂D₃, phosphate, and Ca²⁺ concentrations of wild-type mice and kl/kl mice.

(A–C) Arithmetic means ± SEM of (A) plasma 1,25(OH)₂D₃ (n = 6), (B) phosphate (n = 12), and (C) Ca²⁺ (n = 12) concentrations of wild-type mice (WT, white bars) and kl/kl mice (black bars) either untreated, treated with NH₄Cl solution (280 mM in drinking water) or treated with NH₄Cl and vitamin D deficient diet (LVD, right bars). ***(p < 0.001) indicates statistically significant differences from respective wild-type mice (WT); ^#(p < 0.05), ^##(p < 0.01), ^###(p < 0.001) indicates statistically significant differences from untreated kl/kl mice; ^§§(p < 0.01), ^§§§(p < 0.001) indicates statistically significant differences from respective NH₄Cl treated mice on control diet. (ANOVA).