Figure 3. Effect of NH₄Cl treatment and low vitamin D diet on plasma pai-1and corticosterone levels.

(A) Arithmetic means ± SEM (n = 8, ♂ = 4, ♀ = 4) of plasma pai-1 concentrations in wild-type mice (WT, white bars) and kl/kl mice (black bars) either untreated, treated with NH₄Cl solution (280 mM in drinking water) or treated with NH₄Cl and a vitamin D deficient diet (LVD, right bars). *(p < 0.05) indicates statistically significant differences from untreated wild-type mice (Control); ^##(p < 0.01) indicates statistically significant differences from NH₄Cl treated kl/kl mice on control diet. (ANOVA).(B) Arithmetic means ± SEM (n = 12, ♂ = 6, ♀ = 6) of plasma corticosterone concentrations of wild-type mice (WT, white bars) and kl/kl mice (black bars) either untreated, treated with NH₄Cl solution (280 mM in drinking water) or treated with NH₄Cl and a vitamin D deficient diet (LVD, right bars). Blood was drawn between 4 p.m. and 6 p.m.