Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Apr 20;7:11407. doi: 10.1038/ncomms11407

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2016, Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved.

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

(a) Domain organization of the SKAP:Astrin complex. Both Astrin and SKAP consist of three domains: a mainly unstructured N-terminal domain followed by two coiled-coil domains. (b) Solubility of Astrin and SKAP constructs in isolated and co-expression in bacteria and insect cells. NT, not tested. Samples not marked by asterisks were purified by means of affinity tags that were later removed proteolytically, as discussed in Methods section. (c) Representative electron micrographs of negative-stained SKAP^159–316:Astrin^482–850 complex. Scale bar, 10 nm. (d) Summary of microtubule-binding properties of Astrin and SKAP constructs in co-sedimentation assays with purified isolated or combined constructs. NT, not tested. (e) Representative SDS–PAGE of microtubule co-sedimentation assay with 0-10 μM taxol-stabilized microtubules (MTs) and 1 μM SKAP^159–316:Astrin^482–850 complex. M, molecular weight marker; P, pellet fraction; S, soluble fraction. Images originate from two different gels as indicated by dividing lines. (f) Representative SDS–PAGE of microtubule co-sedimentation assay with 0-10 μM taxol-stabilized MTs and 1 μM SKAP^159–316. M, molecular weight marker; P, pellet fraction; S, soluble fraction. Images originate from two different gels as indicated by dividing lines. (g) Representative SDS–PAGE of microtubule co-sedimentation assay with 0-10 μM taxol-stabilized MTs and 1 μM SKAP^135–225. M, molecular weight marker; P, pellet fraction; S, soluble fraction. Images originate from two different gels as indicated by dividing lines. (h) Quantification and fitting analysis of microtubule co-sedimentation assays shown in (e–g; mean±s.e.m., n≥3).