Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Optimal number of reference genes required for accurate normalization in all three experimental groups using the geNorm tool. The pairwise variation (Vn/Vn+1) was calculated by geNorm tool to determine the minimum number of reference genes for accurate normalization in each experimental set. The cutoff value was 0.15, below which additional reference genes are not necessary for gene expression normalization. The dotted line indicates the optimal number of reference genes.
