Table 2.

The stability ranking of 15 candidate Sorghum bicolor reference genes in three experimental sample sets calculated by delta Ct (DCT), geNorm (GN), NormFinder (NF), BestKeeper (BK) and RefFinder (RF) algorithms.

Rank	All Samples					Abiotic stress					Tissues
	DCT	GN	NF	BK	RF	DCT	GN	NF	BK	RF	DCT	GN	NF	BK	RF
1	PP2A	PP2A	PP2A	EF-P	PP2A	PP2A	CYP	PP2A	GAPDH	PP2A	EIF4α	SAMDC	EIF4A	EF-P	EIF4α
2	ACP2	EIF4α	EIF4α	MACP	EIF4α	CYP	PP2A	CYP	EF-P	CYP	SAMDC	PP2A	PP2A	PP2C	PP2A
3	EIF4α	ACP2	ACP2	PP2C	ACP2	PP2C	EF-P	α-TUB	PP2A	EF-P	PP2A	EIF4α	SAMP	CYP	SAMDC
4	MACP	SAMDC	MACP	6PGDH	MACP	EF-P	MACP	MDH	CYP	PP2C	ACP2	ACP2	ACP2	6PGDH	ACP2
5	SAMDC	ADP-RF	SAMDC	CYP	EF-P	MDH	PP2C	PP2C	EIF4α	α-TUB	6PGDH	6PGDH	6PGDH	MACP	6PGDH
6	6PGDH	6PGDH	6PGDH	EIF4α	6PGDH	α-TUB	ACP2	EF-P	MACP	MACP	MACP	ADP-RF	MACP	ACP2	EF-P
7	EF-P	EF-P	PP2C	PP2A	SAMDC	MACP	MDH	MACP	α-TUB	MDH	ADP-RF	EF-P	β-TUB	EIF4α	MACP
8	ADP-RF	MACP	EF-P	ACP2	PP2C	ACP2	α-TUB	ACP2	ACP2	GAPDH	EF-P	MACP	A-TUB	UBC1	PP2C
9	MDH	MDH	ADP-RF	ADP-RF	ADP-RF	EIF4α	ADP-RF	EIF4α	MDH	ACP2	β-TUB	MDH	ELFP	PP2A	ADP-RF
10	PP2C	α-TUB	MDH	SAMDC	MDH	ADP-RF	EIF4α	SAMDC	ADP-RF	EIF4α	α-TUB	α-TUB	ADPRF	SAMDC	CYP
11	α-TUB	PP2C	α-TUB	MDH	CYP	SAMDC	SAMDC	ADP-RF	PP2C	ADP-RF	MDH	B-TUB	PP2C	β-TUB	β-TUB
12	β-TUB	β-TUB	β-TUB	UBC1	α-TUB	6PGDH	6PGDH	6PGDH	SAMDC	SAMDC	PP2C	PP2C	MDH	ADP-RF	α-TUB
13	CYP	CYP	CYP	α-TUB	β-TUB	GAPDH	GAPDH	GAPDH	6PGDH	6PGDH	CYP	UBC1	CYP	MDH	MDH
14	UBC1	UBC1	UBC1	β-TUB	UBC1	UBC1	UBC1	UBC1	UBC1	UBC1	UBC1	CYP	UBC1	α-TUB	UBC1
15	GAPDH	GAPDH	GAPDH	GAPDH	GAPDH	β-TUB	β-TUB	β-TUB	β-TUB	β-TUB	GAPDH	GAPDH	GAPDH	GAPDH	GAPDH