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Additional metabolites showing different patterns of abundance between young and elderly groups. NAD+ (A), NADP+ (B), leucine (C), and isoleucine (D) showed higher levels in the youth. N6-acetyl-lysine is higher in elderly people (E). Peak areas of metabolites were divided into 10 bins in each group. Error bars represent means ± SD. The range of P values was 0.0017–0.046.
