Table 2.

Histopathology summary

Histopathology findings	Controls (untreated)	J199-treated groups
		Day 6+10	Day 7+11	Day 9+12
Succumbed/euthanized on:	Day 13 or 14	Day 40	Day 40	Day 21
Pathology findings
Hepatocellular vacuolar degeneration	3/3	3/6	1/5	NT
Hepatocellular necrosis	3/3	1/6	1/5	NT
Kupffer cell hyperplasia	3/3	6/6 (mild)	5/5 (mild)	NT
Splenic lymphoid depletion	3/3	0/6	0/5	NT
Lymphoplasmacytic meningoencephalitis and diffuse gliosis*	0/3	5/6	5/5	2/2 (mild)
Diffuse gliosis only	3/3	1/6	0/5	0/2
Immunohistochemistry findings
JUNV antigen detected in liver	3/3	0/6	0/5	NT
JUNV antigen detected in spleen	3/3	0/6	0/5	NT
JUNV antigen detected in brain (neurons, often associated with glial nodules)	3/3	0/6	3/5	2/2

^*Control animals evaluated histologically at earlier time points (day 13 or 14) uniformly had diffuse gliosis only, indicating an initial response of the neural tissues to the JUNV virus but not enough time to recruit a peripheral inflammatory response to the neural tissues. Most animals evaluated histologically at a later time point (day 21 and day 40) show the ability to recruit a peripheral inflammatory response manifesting as inflammation in the meninges and cuffing of vessels within the brain parenchyma (lymphoplasmacytic meningoencephalitis). In treated animals, however, those treated on day 6+10 lack JUNV antigen, whereas those treated at day 7+11 have approximately half of the animals, and all of the animals treated at day 9+12 showing JUNV antigen associated with the inflammation. NT, not tested.