Fig. S5.

The ureC mutant does not have an in vitro growth defect. The ureC mutant has identical growth kinetics in LB medium compared with the wild-type parent strain HI4320, consistent with its initial report (53). The same was true when these strains were cultured in minimal medium, even if the sole nitrogen source, ammonium sulfate, was halved. Results are from three independent experiments. Although the ureC mutant is readily cultured in pooled human urine (28), the parent strain rapidly generates alkaline conditions (pH > 9.0) with consequent growth inhibition and mineral precipitation.