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. 2016 Apr 5;113(16):4326–4331. doi: 10.1073/pnas.1522372113

Fig. S3.

Fig. S3.

Validation of anti-R30 asMe2 antibodies. (A) Dot-blot analysis of unmodified, monomethylated, or asymmetric dimethylated RelA peptides (amino acids 26–34) using anti-R30 asMe2 antibodies. (B) Unmodified or monomethylated RelA peptides (amino acids 26–34) were left unprocessed or were methylated by PRMT1 or PRMT5 in vitro and analyzed as above. The same amounts of unmodified, monomethylated, or asymmetrically dimethylated RelA peptides served as negative or positive controls, respectively (Right). Distinct parts of the same nitrocellulose membrane are separated by a white space.