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(A) Immunoblot analyses of ectopically expressed RelA mutants using anti–c-myc (9E10) antibody. The loading was controlled by anti–α-tubulin antibody. (B) Comparison of nonmethylated and methylated proteins used in the EMSA. Comparable amounts of nonmethylated and methylated proteins were verified by immunoblotting with anti-6×His/HRP antibody.
