Structure-based sequence alignment of the eukaryotic transcription and/or replication terminator family. Secondary structure elements are shown above the sequence and color-coded according to Fig. 1 with MybAD1(αA–αD), MybAD2(αA′–αD′), MybR1(α1–α3), MybR2(α4–α6), and TTD(αE–αI). Sequence coloring highlights regions that are highly conserved (red) and moderately conserved (orange). Reb1_Sp, S. pombe Reb1; RTF1_Sp, S. pombe replication termination factor 1; Nsi1_Sc, S. cerevisiae NTS1 silencing protein 1 also known as Ydr026c; Reb1p_Sc, S. cerevisiae Reb1 protein; TTFI_Hs, Homo sapiens transcription termination factor I; TTFI_Mm, Mus musculus transcription termination factor I; Rib2_Xt, X. tropicalis.