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. 2016 Mar 28;113(16):E2267–E2276. doi: 10.1073/pnas.1525465113

Table S3.

Yeast strains and plasmids used in this study

Yeast strains and plasmids Genotype Source
S. pombe strains
 SP976 h+ ade6-M210 Ura4-D18 leu1-32 (38)
 SPGK100 h+ ade6-M210 Ura4-D18 leu1-32 reb1Δ:: kanMX Lab stock
S. cerevisiae
 PJ69-4A MATa trp1-Δ901 leu2-3,112 901 ura3-52 his3-Δ200 gal4Δ gal8Δ (65)
GAL2-ADE2 LYS2::GAL1-HIS3 met2::GAL7-lacZ
Plasmids
 pREP41-FReb1 pREP41-146- Full-length Reb1 cloned in pREP41 vector This study
 504Reb1 pREP41- 146–504 Reb1 cloned in pREP41 vector This study
 Reb1Δ418 pREP41- 1–418 Reb1 cloned in pREP41 vector This study
 FReb1 Full length Reb1(W 460R-L485P) cloned in pREP41 vector This study
 pGBT9-FReb1 Full length Reb1 cloned in pGBT9 vector This study
 pGBT9-146–504Reb1 146–504 Reb1 cloned in pGBT9 vector This study
 pGBT9-1–145Reb1 1–145 Reb1 cloned in pGBT9 vector This study
 pGAD-146–504Reb1 146–504 Reb1 cloned in pGAD424 vector This study
 pGAD-419–504Reb1 419–504 Reb1 cloned in pGAD424 vector This study
 pGAD-W460R-L485PReb1 419–504 Reb1(W460R- L485P) cloned in pGAD424 vector This study
 pGBKT7Rpa12 Rpa12 cloned in pGBKT7 vector This study
 pMAL C Kin 1–145Reb1 1–145 Reb1 cloned in pMAL C kinase vector This study
 pMAL C Kin 146–504Reb1 146–504 Reb1 cloned in pMAL C kinase vector This study
 pMAL C Kin 419–504Reb1 419–504 Reb1 cloned in pMAL C kinase vector This study
 Reb1 (W460R- L485P) 419–504 Reb1(W460R-L485P) cloned in pMAL This study
 pGEX2TK Rpa12 Rpa12 cloned in pGEX2TK vector This study