Table S3.
Yeast strains and plasmids used in this study
| Yeast strains and plasmids | Genotype | Source |
| S. pombe strains | ||
| SP976 | h+ ade6-M210 Ura4-D18 leu1-32 | (38) |
| SPGK100 | h+ ade6-M210 Ura4-D18 leu1-32 reb1Δ:: kanMX | Lab stock |
| S. cerevisiae | ||
| PJ69-4A | MATa trp1-Δ901 leu2-3,112 901 ura3-52 his3-Δ200 gal4Δ gal8Δ | (65) |
| GAL2-ADE2 LYS2::GAL1-HIS3 met2::GAL7-lacZ | ||
| Plasmids | ||
| pREP41-FReb1 pREP41-146- | Full-length Reb1 cloned in pREP41 vector | This study |
| 504Reb1 pREP41- | 146–504 Reb1 cloned in pREP41 vector | This study |
| Reb1Δ418 pREP41- | 1–418 Reb1 cloned in pREP41 vector | This study |
| FReb1 | Full length Reb1(W 460R-L485P) cloned in pREP41 vector | This study |
| pGBT9-FReb1 | Full length Reb1 cloned in pGBT9 vector | This study |
| pGBT9-146–504Reb1 | 146–504 Reb1 cloned in pGBT9 vector | This study |
| pGBT9-1–145Reb1 | 1–145 Reb1 cloned in pGBT9 vector | This study |
| pGAD-146–504Reb1 | 146–504 Reb1 cloned in pGAD424 vector | This study |
| pGAD-419–504Reb1 | 419–504 Reb1 cloned in pGAD424 vector | This study |
| pGAD-W460R-L485PReb1 | 419–504 Reb1(W460R- L485P) cloned in pGAD424 vector | This study |
| pGBKT7Rpa12 | Rpa12 cloned in pGBKT7 vector | This study |
| pMAL C Kin 1–145Reb1 | 1–145 Reb1 cloned in pMAL C kinase vector | This study |
| pMAL C Kin 146–504Reb1 | 146–504 Reb1 cloned in pMAL C kinase vector | This study |
| pMAL C Kin 419–504Reb1 | 419–504 Reb1 cloned in pMAL C kinase vector | This study |
| Reb1 (W460R- L485P) | 419–504 Reb1(W460R-L485P) cloned in pMAL | This study |
| pGEX2TK Rpa12 | Rpa12 cloned in pGEX2TK vector | This study |