Fig. S4.

Dual cediranib+MEDI3617 therapy does not affect tumor apoptosis or proliferation. Gl261 (A and B) and U87 (C and D) tissues were collected from mice treated with control (green bars), MEDI3617 (orange bars), cediranib (red bars), or dual therapy (blue bars) at various time points after beginning treatment. Sections were stained for cleaved caspase 3 (apoptosis) or Ki67 (proliferation) and DAPI (nuclei). (A and B) In Gl261 tissue, neither apoptosis (A) nor proliferation (B) was altered by dual therapy at days 14 or 21. (C and D) Similarly, in U87 tissue dual therapy had no effect on apoptosis (C) or proliferation (D). Error bars represent the SEM.