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. 2016 Apr 4;113(16):4470–4475. doi: 10.1073/pnas.1525349113

Fig. S8.

Fig. S8.

Peritoneal macrophage gene expression is altered by dual therapy. Peritoneal macrophages isolated from C57BL/6 mice were untreated (control; green bars) or were treated with MEDI3617 (100 nM) (orange bars), cediranib (0.5 μM) (red bars), or dual therapy (blue bars) for 24 h. (A) Gene-expression analysis showed that the M1-associated genes Cxcl9 (P = 0.011), Cxcl11 (P = 0.033), and Tnfα (P < 0.0001) were more highly expressed in dual therapy-treated macrophages than in untreated macrophages. Furthermore, dual therapy also increased the expression of Cxcl9 (P = 0.011), Cxcl11 (P = 0.020), and Tnfα (P = 0.0002) compared with MEDI3617 treatment. Finally, Cxcl9 (P = 0.011), Cxcl11 (P = 0.034), and Tnfα (P = 004) also were more highly expressed in dual therapy-treated macrophages than in cediranib-treated macrophages. (B) Gene expression analysis showed that the M2-associated genes did not change significantly in any of the treatments. Samples were measured in duplicate and experiments were repeated twice (n = 2 per treatment group). *P < 0.05, **P > 0.01, ***P > 0.001.