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Example images of stepwise labeling reactions using model DNA. Positive controls are synthetic DNA bearing biotin and fluorophore. Top row labeled sample is biotin and Cy3 end-labeled genomic DNA fragments. Bottom row labeled sample is synthetic DNA bearing 5hmC and biotin after labeling reaction. Negative controls are synthetic DNA bearing biotin incubated with fluorophore without the enzymes. All DNA concentrations are 30 pM.
