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An absorbance spectrum of a 36mer synthetic DNA bearing one 5mC and one 5hmC after the dual labeling reaction in which 5mC is labeled with Cy3 and 5hmC is labeled with Cy5. The absorption peaks at 260, 555, and 647 nm correspond to the absorption maxima of DNA, Cy3, and Cy5, respectively. The concentrations are calculated using the extinction coefficients of the synthetic DNA, Cy3, and Cy5.
