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Methionine-starved HMDMs were cultured with 10 μCi/mL [35S]methionine ± activation (with 3 μg/mL CD40L and 5 ng/mL IFNγ) and ± addition of HNP1 (25 μg/mL) for 4 or 18 h. (A) TNFα in the culture supernatants at 18 h. (B) Total intracellular and secreted proteins are shown in these Gelcode blue stained gels. Results are representative of experiments repeated three times. Tukey’s post hoc test following a one-way ANOVA. **P < 0.005.
