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. 2016 Apr 4;113(16):4476–4481. doi: 10.1073/pnas.1525360113

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Fig. S7. — Treatment with B20 and A2V skews macrophages toward the M1 phenotype in Gl261 tumors. In all panels, IgG, n = 6; B20, n = 6; A2V, n = 5. (A and B) Gating through CD45^high/CD11b⁺ (A) and F4/80⁺/GR1⁻ (B) identified macrophages. (C) The total percentage of macrophages was not changed by treatment. (D) M1 macrophages were increased by A2V treatment compared with IgG treatment (*P = 0.05). (E) M2 macrophages were reduced by treatment with A2V compared with IgG treatment (*P = 0.05). (F) The M1/2 macrophage ratio trended toward an increase in M1 under A2V treatment as compared with IgG treatment. (G–I) Representative gates for IgG (G), B20 (H), and A2V (I).