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. 2016 Apr 4;113(16):4476–4481. doi: 10.1073/pnas.1525360113

Fig. S9.

Fig. S9.

Gene expression in TAMs isolated from Gl261 tumors suggests a treatment-induced increase in M1 function-related genes and decreased expression of M2 TAM-associated genes. In all panels, IgG, n = 3; B20, n = 3; A2V, n = 3. (A and B) Gl261 TAMs were identified by gating through CD45 and CD11b (A) and further gating through CD11b and F4/80 (B). (C) Cxcl9 gene expression was increased in B20-treated (****P = <0.0001) and in A2V-treated (***P ≤ 0.0001) tumors as compared with IgG-treated tumors. Il6 expression was increased in B20-treated tumors compared with control (**P = 0.003). Compared with control, expression of the M2 marker CD206 was reduced by A2V treatment (*P = 0.03). Gene expression was normalized to gene expression levels in IgG-treated tumors (dotted horizontal line).