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. 2016 Apr 4;113(16):4476–4481. doi: 10.1073/pnas.1525360113

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Fig. S12. — Treatment with B20 or A2V increases M1 microglia in MGG8 tumors. In all panels, IgG, n = 4; B20, n = 7; A2V; n = 9. (A and B) Microglia were defined as CD45^low/CD11b⁺/F4/80⁺/GR1⁻. (C) The total percentage of microglia trended toward an increase under treatment with either B20 or A2V compared with control. (D) A2V treatment increased M1 microglia compared with IgG treatment (***P ≤ 0.001). B20 increased M1 microglia compared with IgG treatment (*P = 0.01). (E) The presence of M2 microglia trended toward a decrease with A2V treatment compared with IgG treatment. (F) There was a trend toward an increased M1/M2 microglia ratio under treatment with A2V compared to IgG. (G–I) Representative gates for IgG (G), B20 (H), and A2V (I).