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(A) Relative level of long-chain CoA thioesters in Sirt5 KO hearts compared with WT after 24 h of fasting (mean ± SEM, n = 3 per genotype, **P < 0.05, *P < 0.1). (B) Relative level of odd-chain acyl-CoAs in Sirt5 KO hearts compared with WT after 30 min of exercise (mean ± SEM, n = 3 per genotype, *P < 0.1). (C) Citrate synthase from Sirt5 KO mouse heart had increased succinylation.
