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Extension of mismatched DNA with Vent pol. Starting with DNA22 (G•T), dTTP was added to the reaction with Vent pol to determine whether the pol was able to bind the mismatched substrate. Within 30 s, a significant amount of the primer was correctly extended past the mismatch. Within 60 min, the pol was able to not only fully extend the initial mismatched DNA, but also misincorporate dTTP opposite the templating dT.
