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Effect of increasing ionic strength on pyrophosphorolysis activity. (A) Correctly matched p/t terminus. Increasing the amount of (NH4)2SO4 present in the reaction buffer results in a decrease in pol activity, although considerable pyrophosphorolysis is still observed at a fivefold increase. (B) Incorrectly matched p/t terminus. No pyrophosphorolysis activity is observed, even at 48 h, upon increasing (NH4)2SO4.
