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. 2016 Apr 4;113(16):E2277–E2285. doi: 10.1073/pnas.1600279113

Table 1.

Summary of results of apparent free energy calculations

Primer/template	Reaction^*		Apparent $Δ G_{inc}^{°}$ ^†, kcal/mol	Apparent $Δ G_{pyro}^{°}$ ^‡, kcal/mol
…CACCG₂₁	Vent_inc	+dGTP	−1.19 ± 0.10
…GTGGCTATCTT…	Vent_inc	+dGTP	−1.19 ± 0.10
…CACCGG₂₂	Vent_pyro	+dGTP		> +7.5^§
…GTGGCTATCTT…	Vent_pyro	+dGTP		> +7.5^§
…CACCG₂₁	Vent_inc	+dATP	−5.22 ± 0.05
…GTGGCTATCTT…	Vent_inc	+dATP	−5.22 ± 0.05
…CACCGA₂₂	Vent_pyro	+dATP		+5.51 ± 0.09
…GTGGCTATCTT…	Vent_pyro	+dATP		+5.51 ± 0.09
…CACCG₂₁	Pfu_inc	+dGTP	−0.21 ± 0.11
…GTGGCTATCTT…	Pfu_inc	+dGTP	−0.21 ± 0.11
…CACCGG₂₂	Pfu_pyro	+dGTP		+5.89 ± 0.13
…GTGGCTATCTT…	Pfu_pyro	+dGTP		+5.89 ± 0.13
…CACCG₂₁	Pfu_inc	+dATP	−5.41 ± 0.15
…GTGGCTATCTT…	Pfu_inc	+dATP	−5.41 ± 0.15
…CACCGA₂₂	Pfu_pyro	+dATP		+6.03 ± 0.32
…GTGGCTATCTT…	Pfu_pyro	+dATP		+6.03 ± 0.32

Values are reported as mean ± SD.

^*

Pyrophosphate is present in all reactions.

^{^†}

Inc indicates the reaction begins with 21mer primer and dNTP is incorporated by polymerization.

^{^‡}

Pyro indicates the reaction begins with a 22mer primer and dNTP is removed by pyrophosphorolysis.

^{^§}

Vent is unable to perform pyrophosphorolysis on mismatched p/t; therefore the value given is based on the detection limit of the assay.