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. Author manuscript; available in PMC: 2016 Aug 27.
Published in final edited form as: Cell. 2015 Aug 27;162(5):961–973. doi: 10.1016/j.cell.2015.07.056

Figure 3. Activation of interferon responsive genes by transient low dose treatment of 5-AZA-CdR is dependent on MDA5/MAVS/IRF7 activation.

Figure 3

LIM1215 colorectal cancer cell line with or without shRNA against RIG1 (A), MDA5 (A), or MAVS (B) was transiently treated with 5-AZA-CdR. Gene expression of four selected interferon responsive genes was measured by real-time qPCR at day 5. C) Upstream Regulators Analysis using Ingenuity Pathway Analysis reveals a significant overlap between Group 4 genes and IRF7 direct target genes (p-value: 5.09e-15). D) Confocal microscopy of LIM1215 cells after treatment with transient low dose of 5-AZA-CdR. Total IRF7 is stained in green, and nuclei are stained in blue (DAPI). There is increased IRF7 expression and nuclear translocation in 5-AZA-CdR treated WT cells. E) LIM1215 colorectal cancer cell line with or without shRNA against IRF7 was transiently treated with 5-AZA-CdR. Gene expression of four selected interferon responsive genes was measured by real-time qPCR at day 5. Y-axis represents the relative expression (in % of control) compared to the wild-type untreated control. Error bars represent the SD of at least three independent experiments. ns, non-significant; * p<0.05; ** p<0.01; **** p<0.0001 (One-way ANOVA). See also Figure S3.