Skip to main content
. Author manuscript; available in PMC: 2016 Aug 27.
Published in final edited form as: Cell. 2015 Aug 27;162(5):961–973. doi: 10.1016/j.cell.2015.07.056

Figure 5. Transient 5-AZA-CdR treatment prolonged effect on cell growth and self-renew is dependent on MDA5/MAVS/IRF7 activation.

Figure 5

A-C) Population doubling time of LIM1215 with or without MDA5 (A), MAVS (B), and IRF7 (C) knockdown. The y-axis denotes population-doubling time (in hours) as a percentage of the vehicle-treated WT controls. The x-axis represents time (in days) after 5-AZA-CdR was withdrawal. Error bars represent the SD of three independent experiments. D) Schematic representation of the approach used to measure the sphere-initiating frequency after transient low dose 5-AZA-CdR treatment (see Experimental Procedures). E-F) Frequency of LIM1215 CICs before and after low dose transient 5-AZA-CdR treatment measured by in-vitro limiting dilution assay in wild-type (E) or shMAVS (F) cells. The y-axis denotes the confidence intervals (lower, estimate and upper) for CIC frequency. G) Schematic representation of the approach used to measure the cancer-initiating cells frequency in vivo after transient low dose 5-AZA-CdR treatment (see Experimental Procedures). H-I) Frequency of LIM1215 CICs before and after low dose transient 5-AZA-CdR treatment measured by in-vivo limiting dilution assay in wild-type (H) or shMAVS (I) cells. The y-axis denotes the confidence intervals (lower, estimate and upper) for CIC frequency. * p<0.05; ** p<0.01; *** p<0.001. Multiple t tests were used to test for difference in population doubling time. Pairwise chi-squared test was used to test for difference in CIC frequency. See also Figure S5.