Table 2.
Results of LDLR+/− Pig Generation (by Somatic Cell Nuclear Transfer)
| Donor Cell Type | No. Reconstructed Oocytes | No. (%) Embryos Transferreda | No. Recipients | No. (%) Pregnancies by Day 30b | No. (%) Fetusesc | No. (%) Term Pregnanciesb | No. (%) Bornc | No. (%) Survivedd |
|---|---|---|---|---|---|---|---|---|
| G418‐resistant fetal fibroblaste | 2967 | 1290 (43.5)f | 6 | 2 (33.3)g | 9 (0.7) | — | — | — |
| Fetal fibroblast from targeted cloneh | 6351 | 2509 (39.5)f | 11 | — | — | 6 (50) | 31 (1.6)i | 2 (6.5) |
a
Expressed as percentage of surviving reconstructed oocytes.
b
Expressed as percentage of recipients.
c
Expressed as percentage of transferred embryos.
d
Pigs that survived for longer than 7 months; expressed as percentage of live‐born piglets.
e
PCR‐positive colonies obtained after TV electroporation and subsequent G418 selection.
f
Embryos that had developed to the 2‐ to 8‐cell stage after 2 days of culture in vitro were transferred to recipients.
g
Pregnancies were terminated on days 39 and 72 for isolation of embryonic fibroblasts.
h
Cells isolated from a cloned fetus in which targeting was confirmed.
i
Eight stillborn piglets not included.