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. Author manuscript; available in PMC: 2016 Apr 25.
Published in final edited form as: J Am Chem Soc. 2015 Jul 15;137(29):9302–9314. doi: 10.1021/jacs.5b03524

Figure 11.

Figure 11

Oxidation of p-anisidine as followed by UV–vis absorption spectroscopy. (A) p-Anisidine was added anaerobically to biferrous 3His-G4DFsc(Mut3) followed by addition of O2-saturated buffer. (B) p-Anisidine and O2-saturated buffer were added simultaneously to biferrous 3His-G4DFsc(Mut3). (C) p-Anisidine was added to biferric 3His-G4DFsc(Mut3) (buffer exchanged prior to p-anisidine addition to eliminate any presence of H2O2 in solution). (A) and (B) are data collected over 180 min. Data taken at 20 °C. Final samples contained 65 μM protein and 1 mM substrate.