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. Author manuscript; available in PMC: 2016 Apr 25.
Published in final edited form as: J Am Chem Soc. 2015 Jul 15;137(29):9302–9314. doi: 10.1021/jacs.5b03524

Figure 15.

Figure 15

NIR CD spectra of the p-anisidine reaction with 3His-G4DFsc(Mut3). (A) CD spectra of the 3His protein in the presence of p-anisidine before exposure to O2 (green), under aerobic conditions (teal), and 30 min after first allowing O2 containing buffer (~250 μM O2) to react for 60 s and then sealing the CD cell to create a closed system (red). Protein concentrations for red and teal are 250 μM. (B) Time dependence of the 10 500 cm−1 feature after first allowing 3His-G4DFsc(Mut3)+P-AN to react with O2 followed by sealing the CD cell to create a closed system (see red in (A)). Data collected at 20 °C.