VTVH MCD data for biferrous G4DFsc and 3His-G4DFsc(Mut3) following 4-aminophenol (4-AP) addition. (A) Overlay of the VTVH MCD for G4DFsc (purple) (at 9100 cm−1) and 3His-G4DFsc(Mut3) (orange) (at 10 400 cm−1) with addition of 4-AP. (B) Doublet fit to the VTVH MCD of 3His-G4DFsc(Mut3)+4-AP. Isotherms at 2, 3, 5, 7.5, 10, 15, and 20 K are shown.