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. Author manuscript; available in PMC: 2016 Apr 25.
Published in final edited form as: J Am Chem Soc. 2015 Jul 15;137(29):9302–9314. doi: 10.1021/jacs.5b03524

Figure 9.

Figure 9

Biferrous CD features during the 4-aminophenol (4-AP) oxidation reaction for 3His-G4DFsc(Mut3). (A) CD of 3His-G4DFsc-(Mut3)+4-AP before (red) and after (green) addition of O2-saturated buffer. (B) The time course over 160 min of the normalized intensity of the CD feature at 10 600 cm−1 (red line is the kinetic fit). CD data collected at 4 °C in the presence of ~15-fold excess substrate (~0.25 mM protein).