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. 2016 Apr 18;5(4):e003327. doi: 10.1161/JAHA.116.003327

Figure 2.

Figure 2

Effect of ADMA on simvastatin‐mediated promotion of NO production, eNOS phosphorylation, and angiogenesis. A and B, Human aortic endothelial cells (HAECs) were pretreated with SDMA (0.125, 0.25, 0.5, 1.0, and 2.0 μmol/L) or ADMA (0.125, 0.25, 0.5, 1.0, and 2.0 μmol/L) for 30 minutes, then simvastatin (10 μmol/L) for 24 hours. Levels of nitrite in culture media and intracellular cGMP were measured by Griess assay and ELISA kits. C, After preincubation with ADMA, HAECs were treated with or without simvastatin (10 μmol/L) for 10 minutes. Western blot analysis of phosphorylated eNOS (p‐eNOS) at Ser1179 and total eNOS level. D, Tube formation by HAECs; bar graphs indicate the fold increase in number of branch points in 5 randomly selected microscopy views. *P<0.05 vs vehicle; # P<0.05 vs simvastatin alone. ADMA indicates asymmetric dimethylarginine; eNOS, endothelial nitric oxide synthase; NO, nitric oxide; SDMA, symmetric dimethylarginine.