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. 2015 Aug 12;48(2):473–482. doi: 10.4143/crt.2015.116

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Copyright © 2016 by the Korean Cancer Association

This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-CommercialLicense (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 1. — SOX2 amplification was assessed using fluorescence in situ hybridization (FISH, ×1,000) and protein expression was determined using immunohistochemistry (×200) in lung squamous cell carcinoma patients. SOX2-specific DNA probe in green combined with a centromere 3-specific probe in red was applied for FISH. (A) Nucleus without SOX2 amplification. (B) Nucleus with low-level SOX2 amplification (arrows). (C) Nucleus with high-level SOX2 amplification (arrows). (D) Moderate nuclear SOX2 expression (arrow). (E) Strong nuclear SOX2 expression (arrow). (F) Weak nuclear SOX2 expression.