Morphological changes of DUOC-01 cells during manufacturing. In each row, elapsed time from the image on the right is indicated and an arrow shows a single cell in different frames. (A, F, G) Spread-out cells rounding up to become a motile cell. (B, C) Cell shape changing from elongated to round. (D, E) Motile refractile cells attaching to become flat, spread-out cells. (H) A flat cell becoming an elongated, bipolar, motile cell.