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. Author manuscript; available in PMC: 2016 Apr 25.
Published in final edited form as: Cytotherapy. 2015 Mar 12;17(6):803–815. doi: 10.1016/j.jcyt.2015.02.006

Figure 4.

Figure 4

Flow cytometric analysis of DUOC-01. (A) Typical flow forward and side-scatter (FSC, SSC) and viability staining cytograms used to identify viable cells for analysis of marker expression. (B) Flow histogram of gated population in (A) stained with 7-amino-actinmycin (7AAD) (red line) and anti–glycophorin A (GlyA) to identify viable cells and erythrocytes, respectively. (C) Expression of CD11b and CD14 and (D) of CD3 and CD45 by cells in DUOC-01 product. Cells analyzed were gated as shown in (A) and (B). In all cases, fluorescence gates for antibody staining were set by use of cells exposed to appropriate isotype controls.