Table IV.
Concentration of cytokines in medium of DUOC-01 cultures 24 hours after inflammatory stimulation.
| Additions | IL-1b | IL-2 | IL-4 | IL-5 | IL-6 | IL-10 | IL12-p70 | IL-13 | IFN-γ | TNF-α |
|---|---|---|---|---|---|---|---|---|---|---|
| None | OOR< | OOR< | OOR< | OOR< | 181.9 | 27.6 | OOR< | OOR< | OOR< | OOR< |
| SD | 230.7 | 14.6 | ||||||||
| 0.5 mg TNF-α | 14.7 | 43.2 | 7.4 | 19.9 | 4022.5 | 1930.9 | 2.0 | OOR< | 369.2 | NA |
| SD | 2.6 | 2.1 | 0.4 | 1.3 | 414.0 | 356.1 | 0.1 | 15.0 | ||
| 1 μg TNF-α | 16.5 | 47.1 | 8.5 | 20.8 | 7562.6 | 1589.0 | 2.4 | OOR< | 391.2 | NA |
| SD | 3.2 | 4.5 | 0.8 | 1.7 | 1393.1 | 197.4 | 0.3 | 17.9 | ||
| 10 μg IFN-γ | 91.6 | 720.1 | 113.1 | 355.5 | 3596.0 | 780.2 | 344.9 | 103.5 | NA | 169.4 |
| SD | 23.9 | 171.5 | 27.7 | 93.2 | 1000.8 | 172.8 | 94.6 | 27.6 | NA | 30.8 |
| 5 ng PMA | <1.15 | OOR< | OOR< | OOR< | 89.0 | 72.3 | OOR< | OOR< | OOR< | 5.0 |
| SD | 46.8 | 59.8 | 4.8 | |||||||
| Lower limit | 1.1 | 2.8 | 0.5 | 2.6 | 7.3 | 3.5 | 0.6 | 0.9 | 2.7 | 0.1 |
Mean values (expressed in pg/mL) calculated from Bioplex assay immunocapture fluorescence assays from culture supernatants were derived from DUOC cells (manufacturing lots 220055, 900181 and 491255) replated in the presence of inflammatory stimuli for 24 h. Cytokine concentrations were measured in duplicate wells from each lot, and means were calculated from pooled data derived from different lots treated under similar conditions. Lot 220055 was not tested after IFN-γ stimulation.NA, not applicable; this is the cytokine added to stimulate the culture. OOR< means cytokine concentration was below the lower limit of detection is shown in the bottom row.