Figure 9.
AVDCs resist viral infection. A) Barcoding for flow cytometry analysis. Cells were stained with different combinations of 0, 0.3, 1, 4 or 15 μg/ml Pacific Blue–NHS, 0, 1.25, 5 or 20 μg/ml Alexa 350–NHS and 0, 4 or 20 μg/ml Alexa 750–NHS to enable 60 different conditions in one FACS run. B) Time course results for NDV-RFP expression following infection in naïve DC and AVDC. C) Time course for expression of MHC-I, MHC-II and CD86 surface markers in the same cells studied in A and B. The results shown are representative of two independent experiments using cells from different donors.