Figure 5.
Size-exclusion chromatography of PSPF-EKG and PSPF-DKG at pH 5.5 and 7.4. At pH 7.4, both PSPF-EKG and PSPF-DKG produce a single well-behaved peak with an apparent molecular weight corresponding to an aggregation state of n ≈ 6 (B). At pH 5.5, PSPF-EKG produces a single major peak corresponding to n ≈ 5, whereas PSPF-DKG produces only a poorly defined peak (A).