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. 2015 Feb 11;2(2):227–243. doi: 10.1080/23328940.2015.1004972

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© 2015 The Author(s). Published with license by Taylor & Francis Group, LLC

This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

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Figure 3. — Paradigms for assaying thermosensory behavior in Drosophila larvae and adults. (A) Schematic of a circular assay chamber in which flies or larvae must choose between warm and cool (or room temperature and test temperature) regions. The distribution of individuals between these regions can then be used to calculate and avoidance (or preference) index. (B) Schematic of an assay chamber that can be used to test the distribution of individuals across a temperature gradient. The chamber is heated (or cooled) at one end to produce a temperature gradient and divided into zones. The proportional distribution of flies or larvae across these zones can then be calculated. (C) A standard equation used to generate an avoidance index of flies tested in an experimental chamber that requires them to navigate to either room temperature or a test temperature.