Figure 2.

CCL2 does not increase rectal temperature but induces cell migration in rats. (A) Male Wistar rats received an intracerebroventricular injection of 1 μl of saline (SAL) or CCL2 (5, 50, 250, and 500 ng), and body temperature (Tb) was measured by a tele-thermistor probe coupled to a thermometer for 6 h. (B) Male Wistar rats were euthanized 6 h after an intraperitoneal injection of 50 or 500 μg CCL2, and the peritoneal content was collected. Cells from the peritoneal cavity were collected by an injection of 10 ml phosphate-buffered saline. The abdomens were gently massaged, and a blood-free cell suspension was carefully withdrawn with a syringe. The abdominal lavage was placed in plastic tubes, and total cell counts were immediately performed in a Neubauer chamber. The data were analyzed using one way or 2-way repeated-measures analysis of variance, followed by the Bonferroni post hoc test. The data are expressed as the mean ± SEM of the changes in body temperature (A, ΔTb, °C) or number of cells per mm³ (B), and n indicates the number of animals in each group. *p < 0.05, compared with the control group (Soares DM and Souza GEP, unpublished results).