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. 2016 Apr 25;11(4):e0152073. doi: 10.1371/journal.pone.0152073

Fig 2. sCD200 is secreted from CLL cells in response to different stimuli.

Fig 2

CLL cells (data pooled from 6 individual patients) were cultured in AIMV medium and stimulated with a) 40ng/ml PMA; b) 1μM Ionomycin; and c) 3μg/ml Imiquimod with supernatants collected at 48-hr. At 24-hr, cells were stained for CD62L, CD19, and CD200 expressions by FACS (see Fig 3). p-values were obtained from paired t-tests: a) CLL cells from all 6 patients responded to PMA by shedding increased amounts of sCD200 (p = 0.0008), but the degree of response to PMA varied amongst patients. b) CLL cells from 4 out of 6 patients showed a modest response to Ionomycin (p = 0.0532). 2 out of 6 patients showed no response to Ionomycin treatment. c) CLL cells from 4 out of 6 patients showed a modest response to Imiquimod, although the group mean induction of CD200 shedding was not statistically significant (p>0.05).