Fig 3. PMA-induced CD200 shedding is reflected as a loss of CD200 expression from the surface of CLL cells.

CLL cells from 6 patients were cultured in medium alone, or with 40ng/ml PMA, and harvested at 24 hour to assess for CD200 and CD62L expression. Median and mean percent loss of CD200 and CD62L from the surface of CLL cells from all 6 patients in the cohort were expressed relative to control cultured cells, as determined by FACS. Both CD62L and CD200 expression were normalized to CD19 expression on the same cells.