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. 2016 Apr 25;11(4):e0154156. doi: 10.1371/journal.pone.0154156

Fig 4. Effect of RNAi induced loss of osm-9 function on let-60(ga89) induced egg hatching defects and larval arrest.

Fig 4

let-60(ga89) encodes a ts mutant of ras GTPase. The mutant phenotype can be induced at permissive temperatures (16°C) by exposing worms to 300 mM NaCl [14,16]). let-60(ga89) mutants were crossed with uIs60[Punc-119::yfp + Punc-119::sid-1] worms to increase the sensitivity of neurons to RNAi (46). let-60(ga89);uIs60[Punc-119::yfp + Punc-119::sid-1] worms were fed bacteria expressing scrambled (control) or osm-9 dsRNA and maintained at 16°C on agar plates containing either 51 or 300 mM NaCl. Values are means ± S.E. (n = 3–5 experiments with 100–300 eggs). *P<0.006 compared to control worms exposed to 51 mM NaCl. **P<0.005 compared to control worms exposed to 300 mM NaCl.