Table 5. Samples with discrepancies.
| Sample # | Tumoral cells content (%) | Mutant allele frequency (%) | Mutation* | Results | |||
|---|---|---|---|---|---|---|---|
| HRM | SybrGreen | Idylla | IHC | ||||
| 1 | 90 | 84.22 | c.1799T>A p.Val600Glu | wild-type | V600E | V600E-E2-D | V600E |
| 10 | 20 | 1.86 | c.1799T>A p.Val600Glu | wild-type | V600E | V600E-E2-D | V600E |
| 16 | 80 | 58.23 | c.1798_1799delinsAA p.Val600Lys | Mutation on exon 15 | V600K | V600K-R-M | V600E |
| 21 | 20 | - | wild-type | Mutation on exon 15 | wild-type | wild-type | wild-type |
| 28 | 10 | 7.59 | c.1798_1799delinsAA p.Val600Lys | Mutation on exon 15 | V600K | V600K-R-M | wild-type |
| 36 | 80 | 39.44 | c.1799T>A p.Val600Glu | wild-type | V600E | V600E-E2-D | wild-type |
| 39 | 20 | 5.38 | c.1799T>A p.Val600Glu | wild-type | V600E | V600E-E2-D | V600E |
| 40 | 10 | 4.87 | c.1796_1799delInsTAGA p.Thr599_Val600delinsIleGlu | Mutation on exon 15 | wild-type | wild-type | wild-type |
| 47 | 90 | 74.25 | c.1789_1790delinsTC p.Leu597Ser | Mutation on exon 15 | wild-type | wild-type | wild-type |
| 50 | 60 | 37.11 | c.1798_1799GT>AA p.Val600Lys | Mutation on exon 15 | V600K | V600K-R-M | V600E |
*Mutations characterized and confirmed by NGS