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. Author manuscript; available in PMC: 2016 Apr 25.
Published in final edited form as: J Mol Biol. 2015 Feb 7;427(6 Pt B):1413–1427. doi: 10.1016/j.jmb.2015.01.018

Figure 4.

Figure 4

Release of 29KE/31KE from T-cell lines. VSV-G pseudotyped viruses were generated by transfecting 293T cells with the HIV-1 mutants indicated and a VSV-G expression vector. These viruses were normalized by RT assay and used to infect Jurkat and MT4 T-cell lines. 24 h postinfection cells were labelled with 35S Met/Cys for 8 or 16 h, then cell and virus lysates were immunoprecipitated and separated by SDS-PAGE. (a) Jurkat. (b) MT4. (c) Samples from three independent experiments were analyzed by fluorography. Virion capsid, expressed as a percentage of total Gag, was plotted relative to WT, +/− S.E.M.