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Rescue of 29KE/31KE replication in Jurkats. (a) Jurkat T cells were transfected with the HIV-1 mutants indicated. Samples were taken every two days and the cells split 1:3. Samples were assayed for RT activity. (b) Virus supernatants from the peaks of WT and 16EK/29KE/31KE/62QR were collected, normalized by RT assay and used to reinfect Jurkat T cells for a 2nd passage. Replication assays were performed three times, data from a representative experiment are shown.
